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TWISTDX TwistAmp NFO kits

TwistDx品牌國內代理商--默瑞(上海)生物科技有限公司

TwistAmp® 核酸擴增產品(pin),能(neng)夠在(zai)15分(fen)鐘內進行(xing)常(chang)溫(wen)下的單分(fen)子核酸檢(jian)(jian)(jian)測該技(ji)術對硬件設備的要求(qiu)很低,特(te)別(bie)適合(he)用(yong)于體外(wai)(wai)診斷、獸醫、食品(pin)安(an)全(quan)、生物安(an)全(quan)、農業等領域。TwistAmp nfo用(yong)于末(mo)(mo)端檢(jian)(jian)(jian)測,檢(jian)(jian)(jian)測DNA擴增反應. 末(mo)(mo)端三明(ming)治法快速(su)檢(jian)(jian)(jian)測,如側向流試(shi)紙法.除基本組分(fen)之(zhi)外(wai)(wai), nfo核酶(mei),和適合(he)是LF 探(tan)針 形成(cheng)新(xin)的聚合(he)酶(mei)增強底物,無需(xu)任何儀器(qi)。本品(pin)可通過加入(ru)exo探(tan)針作(zuo)為Amp exo kit的替代物,進行(xing)熒光定(ding)量檢(jian)(jian)(jian)測,由(you)于核酶(mei)延緩動態(tai)反應速(su)度,可用(yong)于末(mo)(mo)端電泳檢(jian)(jian)(jian)測。
TwistAmp® nfo
產品(pin)貨號(hao)-TANFO02KIT
產品貨號-TwistAmp® nfo
產品用途(tu)-用于末端(duan)檢(jian)測(ce),檢(jian)測(ce) DNA 復制反應(ying)
配(pei)套(tao)儀器-Twirla

TWISTDX TwistAmp NFO kits假陽性問題解答
Q:What will cause the false-positive results except DNA contamination?
A:For the nfo kits another source of false positives is the dimerization of the two primers and in some cases the labeled primer, and the probe.
When this happens you will have the biotin and FITC on an RPA product that will bind to the Lateral flow strip and bind to the ant-FITC gold particle that will result in a line in the test area on the Lateral Flow Strip.
The primer sequences and probe sequence should be check for possible dimerization and they may need to be modified and screened.
There are some basic rules for PCR primers that also hold true for RPA primers. Namely try to avoid:
1) Long homo-polymer repeats
2) Repetitive sequences
3) Regions that could have lots of secondary structure
4) High (>70%) GC or AT regions
5) Sequences likely to cause primer-primer interaction (use
產品簡介
TwistAmp® 核(he)酸擴增(zeng)產(chan)品,能夠在15分鐘內(nei)進行常溫(wen)下(xia)的(de)單分子(zi)核(he)酸檢測(ce)該(gai)技術對硬件設(she)備的(de)要(yao)求很低,特別適合用于(yu)體外(wai)診斷、獸醫、食(shi)品安(an)(an)全、生(sheng)物安(an)(an)全、農業(ye)等(deng)領域。TwistAmp exo 用于(yu)real-time探針(zhen)檢測(ce). 除基本(ben)組分外(wai), 還含有內(nei)切酶3,減(jian)少擴增(zeng)產(chan)物的(de)末端拷貝量,所以不能用于(yu)電泳分析,但在RPA系統中產(chan)生(sheng)更強的(de)動(dong)態熒光信號,便于(yu)實時(shi)定量。
產品詳情介紹

默瑞(上海)生(sheng)物(wu)科技(ji)有限公司,作為專業(ye)的(de)生(sheng)命科學產(chan)品(pin)推廣與(yu)服務商(shang),以(yi)“創(chuang)新,專業(ye),共贏”的(de)理(li)念,不斷強化服務體(ti)系,提(ti)(ti)升(sheng)客(ke)戶(hu)體(ti)驗,以(yi)莫納生(sheng)物(wu)產(chan)品(pin)為主線,結合國內外(wai)優質產(chan)品(pin)提(ti)(ti)供高標準完(wan)整解決(jue)方案,幫助科研(yan)客(ke)戶(hu)和生(sheng)物(wu)技(ji)術企業(ye)高效創(chuang)新,加速轉化,創(chuang)造更多(duo)優質產(chan)品(pin),提(ti)(ti)升(sheng)社會福祉,實(shi)現多(duo)方共贏
默瑞(rui)生(sheng)物(wu)創立以來,已經陸(lu)續導(dao)入:
TwistDx,Immunostep,101Bio,enzymatics,AATBioquest,KAPABIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優質品牌,逐(zhu)步培育(yu)成為專(zhuan)業(ye)用戶的(de)選擇(ze)。